How does a 4D in vitro platform compare to 3D?
In the ever-evolving landscape of in vitro research, the transition from 3D to 4D is bound to become a paradigm shift. Traditionally, 3D tissue models and testing platforms have represented the most advanced systems for safety and efficacy assessments in vitro. However, REVIVO BioSystems’ “4th dimension”, marked by the mimicking of the blood flow, offers a revolutionary leap in understanding product performance over time.
The dynamic nature of our 4D in vitro platform transcends the limitations of static 3D models, providing a more comprehensive perspective that opens up new horizons for cosmetic and pharmaceutical innovation.
The summary table below highlights the strengths of 4D methodologies with respect to conventional 3D ones.
Greater insights into skin aging with our unique Chronological aging model
In vitro skin models help us to understand the mechanisms of aging, advancing the development of innovative treatments and skin care interventions.
While informative, traditional in vitro models present limitations in reproducing natural biological expressions of significant proteins and gene expressions. This can hinder their ability to provide realistic insights into skin-related phenomena.
With REVIVO BioSystems’ 4D in vitro tissue models that leverage its dynamic organ-on-a-chip technology, these skin models closely resemble native human skin where significant proteins and genes, closer to natural levels in native tissues, are expressed. We possess a skin model capable of mimicking chronological aging independently of oxidative stress, offering a more nuanced insight into the aging process of the skin.
Our 4D skin models are ready in a 'healthy' and 'youthful' state, allowing the aging process to unfold over time. Why is this a game-changer? Following the aging process, it is easy to observe a significant reduction in epidermal thickness in the aged 4D skin model and noticeable significant changes to the expression of a youth marker and proliferation. In contrast, when skin models are reconstructed in static cell culture inserts, they already exhibit certain age-related characteristics (e.g. low expression of youth and proliferation markers). As a result, their potential to provide an appropriate and accurate model for distinct anti-aging product claims can be limited.
The analogy diagram and histological analysis below give a visual representation of these differences.
